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  International Journal of Fundamental Physical Sciences (IJFPS) 

    ISSN : 2231-8186

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IJFPS, Vol 3, No 3, Sep, 2013



Functional analysis of the L Protein of Nipah Virus Using Minigenome System‎


Ali Shahandeh , Amir Bin Rabu



Nipah virus (NiV) was identified in Malaysia in late September 1998 as the etiological agent of an outbreak of acute encephalitis with the high mortality rate in human. NiV polymerase gene (L) encodes RNA-dependent RNA polymerase (RdRp) which is an enzyme required for viral replication. The aim of this study is to investigate the role of the N terminal of polymerase protein in replication of NiV. Based on the extensive deduced amino acid sequence analyses of a number of L proteins of non-segmented negative-strand (NNS) RNA viruses, a cluster of high-homology sequence segments have been identified within the body of the L proteins. The functional characterization of the NiV L polymerase was addressed in this research by generating a series of progressive N- deletions in the cloned gene and testing them in a minigenome assay. Five mutations that delete increasing amounts of the amino terminus of the L protein (20 amino acids) were generated at the amino-terminal. The ability of new recombinants plasmids in viral replication were tested by using minigenome system which is based on an intracellular and plasmid-based replication assay. This system contains an inserted chloramphenicol acetyltransferase (CAT) gene, for readout of the replication of the mini genome directed by wild type or mutant L protein. The results demonstrated that the first 100 amino acids of the NH2-terminal domain spanning a highly conserved motif are directly involved in transcription of the genome RNA. The possible functional significance of the NH2-terminal domain of paramyxovirus L protein is discussed.

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Does One-Way Speed of Light is Constant?‎

DOI: 10.14331/ijfps.2013.330053

Hamid Navaei Rahbar



In all measurement methods, the back and forth motion of light in one-way speed theoretically used in two ‎different assumptions first, when the speed of light is not constant and the second when the speed of light is ‎constant. We measured the speed of light only in one-way direction and according to these measurements, ‎found that the speed of light, does not behave as constant velocity and therefore the special theory of relativity ‎and consequently constancy of the speed of light is not to be true.‎

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On The Absolute Motion in the Inertial Systems‎

DOI: 10.14331/ijfps.2013.330054

‎Alfonso Leon Guillen Gomez



Through a mental experiment, the mechanics’ theory, about of the relative motion, is reexamined because the ‎vacuum, frame of the movement, has a physical reality, which allows test the absolute motion, by the Doppler ‎Effect induced by means of a decelerator of photons, affecting an electromagnetic wave in the direction of the ‎motion of an inertial system. This Doppler does not exist in the nature because the source of the ‎electromagnetic wave is in rest respect to system. But, in each cycle, due to which the wave propagates inside ‎the decelerator, the speed of the system combined with the speed of the wave, respect to vacuum, provokes the ‎induced Doppler. The result is that an observer, inside an inertial system, fully insulated from the outside, may ‎determine whether his system is in rest or in motion and measure its speed, in absolute terms.‎

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Morphology Study of anatase nano-TiO2 for Self-cleaning Coating‎

DOI: 10.14331/ijfps.2013.330055

M. Farahmandjou , P. khalili‎



Titanium dioxide (TiO2) has been widely used in the field of pollutant degradation, virus ‎killer and ‎environment protection. In this paper, the 8 nm TiO2 nanoparticles are first ‎synthesized by TiOSO4.2H2O ‎precursor. The samples were then characterized by x-ray ‎diffraction (XRD) and transmission electron ‎microscopy (TEM) analysis and Raman ‎scattering after heat treatments. The XRD results show the sharp picks ‎of the samples after ‎annealing. The SEM results show that the size of nanoparticles is about 8 nm in diameter. ‎‎Finally Raman scattering pattern of the as-prepared TiO2 indicates the anatase phase‎.‎

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